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Freeze-dried Dengue Virus I、II、III、IV Nucleic Acid Detection Kit(Fluorescence PCR)

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Freeze-dried Dengue Virus I、II、III、IV Nucleic Acid Detection Kit(Fluorescence PCR)

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Introduction

[Product Name]

Freeze-dried Dengue Virus I/II/III/IV Nucleic Acid Detection Kit (Fluorescence PCR)

 

[Packing Size]

20 tests/kit, 50 tests/kit

 

[Intended Use]

This kit is used for qualitative typing detection of denguevirusDENV nucleic acid in suspected patient’s serum sample to help diagnose patients with Dengue fever. The test results obtained by the kit are for clinical reference only. To make final diagnosis, comprehensive analysis of patient’s condition must be performed in conjunction with the patient's clinical manifestations and other laboratory findings.

Dengue fever (DF), which is induced by denguevirus (DENV) infection, is one of the most epidemic arbovirus infectious diseases. Its transmission medium includes Aedes aegypti and Aedes albopictus. DF is mainly prevalent in tropical and subtropical areas. DENV belongs to flavivirus under flaviviridae, and can be classified into 4 serotypes according to surface antigen. Clinical manifestations of DENV infection mainly include headache, fever, weakness, enlargement of lymph node, leukopenia and etc., and bleeding, shock, hepatic injury or even death in severe cases. In recent years, climate change, urbanization, quick development of tourism and other factors have provided more rapid and convenient conditions for transmitting and spreading of DF, leading to constant expansion of epidemic area of DF. Therefore, establishment of easy, specific and rapid etiological diagnosis method of DENV has important significance for clinical diagnosis of DF.

 

[Main Components]

No.

Component (20 tests/kit)

Character

Quantity

Component Description

1

DENV Reaction Buffer

Freeze-dried

1 bottle

dengue virus I/II/III/IV primers, fluorescent probes, reaction buffer, Taq enzyme, reverse transcriptase and UDG enzyme, etc.

2

DENV Positive Control

Freeze-dried

1 vial

Mixture of DENV I/II/III/IV templates

3

DENV Blank Control

Liquid

1 vial, 600μL/vial

DNase/RNase free H2O

4

Reconstituted Solution

Liquid

1 vial, 1mL/vial

DNase/RNase free H2O

 

No.

Component (50 tests/kit)

Character

Quantity

Component Description

1

DENV Reaction Buffer

Freeze-dried

1 bottle

dengue virus I/II/III/IV primers, fluorescent probes, reaction buffer, Taq enzyme, reverse transcriptase and UDG enzyme, etc.

2

DENV Positive Control

Freeze-dried

1 vial

Mixture of DENV I/II/III/IV templates

3

DENV Blank Control

Liquid

1 vial, 600μL/vial

DNase/RNase free H2O

4

Reconstituted Solution

Liquid

1 vial, 1.45mL/vial

DNase/RNase free H2O

 

Note: The components of the kit in different batches are not interchangeable.

 

[Storage Conditions and Shelf Life]

  1. Unopened kit: It should be stored at no higher than 30℃ and protected from light, and the shelf-life is 12 months.
  2. After opening the sealed bag, the lyophilized reagent in the tube should be used immediately.
  3. RT-PCR detection should be performed immediately after reconstitution of the lyophilized reaction buffer, otherwise it should be stored at -25℃ to -15℃ and protected from light. The validity period is 1 week, and repeated freezing and thawing should be avoided ( limited to 2 cycles).
  4. After reconstituting the lyophilized positive control, it should be stored at -25°C to -15°C. The validity period is 1 week, and repeated freezing and thawing should be avoided (limited to 2 cycles).
  5. Transportation conditions: It can be transported stably for 1 month when the temperature is not higher than 37℃.
  6.  See the packaging label for the production date, production batch number and expiration date.

[Applicable Instrument]

Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems(Shanghai Hongshi Medical Technology Co., Ltd. ), LightCycler®480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection System(FQD-96A,Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) , BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System.

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