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Freeze-dried Plasmodium Nucleic Acid Detection Kit (Fluorescence PCR)

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Category:Infectious Diseases

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Freeze-dried Plasmodium Nucleic Acid Detection Kit (Fluorescence PCR)

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Introduction

[Product Name]

Freeze-dried Plasmodium Nucleic Acid Detection Kit (Fluorescence PCR)

 

[Packaging Size]

20 tests/kit, 50 tests/kit

 

[Intended Use]

This kit is used for in vitro qualitative detection of Plasmodium nucleic acid in peripheral blood samples of patients with suspected Plasmodium infection.

Malaria (Mal for short) is caused by Plasmodium, which is a single-celled eukaryotic organism, including Plasmodium falciparum Welch, Plasmodium vivax Grassi & Feletti, Plasmodium malariae Laveran, and Plasmodium ovale Stephens. It is a mosquito-borne and blood-borne parasitic disease that seriously endangers human health. Of the parasites that cause malaria in humans, Plasmodium falciparum Welch is the deadliest. The incubation period of different malaria parasites is different, the shortest is 12-30 days, and the longer one can reach about 1 year. After the paroxysm of malaria, symptoms such as chills and fever may appear. The patients may have anemia and splenomegaly. The serious patients may have coma, severe anemia, acute renal failure which may lead to the death of patients. Malaria is distributed worldwide, mainly in tropical and subtropical regions such as Africa, Central America, and South America.

At present, there are three detection methods: Plasmodium blood smear, antigen detection, and nucleic acid detection.The detection of Plasmodium nucleic acid by isothermal amplification technology has the advantages of rapid detection and simple response, and is suitable for detection in large-scale malaria epidemic areas.

The test result of this kit is not the only indicator for the diagnosis of Plasmodium infection in patients, and must be comprehensively analyzed in combination with the clinical manifestations of patients and other test results.

 

[Main Components]

S/N

Component(20 tests/kit)

Character

Strength

Component Description

1

Mal Reaction Buffer

freeze-dried     

1 bottle

Contains Plasmodium, internal control specific primers, fluorescent probes, Taq enzyme, UDG enzyme, reaction buffer, etc.

2

Mal Positive Control

freeze-dried

1 vial

Mixed with Plasmodium and internal reference template

3

Mal Blank Control

liquid

1 vial, 600μL/vial

DNase/RNase free H2O

4

Reconstituted Solution

liquid

1 vial, 1mL/vial

DNase/RNase free H2O,etc.

 

S/N

Component(50 tests/kit)

Character

Strength

Component Description

1

Mal Reaction Buffer

freeze-dried     

1 bottle

Contains Plasmodium, internal control specific primers, fluorescent probes, Taq enzyme, UDG enzyme, reaction buffer, etc.

2

Mal Positive Control

freeze-dried

1 vial

Mixed with Plasmodium and internal reference template

3

Mal Blank Control

liquid

1 vial, 600μL/vial

DNase/RNase free H2O

4

Reconstituted Solution

liquid

1 vial, 1.45mL/vial

DNase/RNase free H2O,etc.

Note: Components of different batches cannot be mixed or interchanged.

 

 

[Storage Conditions and Shelf-life]

1.Unopened kit: It should be stored at no higher than 30℃ and protected from light, and the shelf-life is 12 months.

2. After opening the sealed bag, the lyophilized reagent in the tube should be used immediately.

3. RT-PCR detection should be performed immediately after reconstitution of the lyophilized nucleic acid reaction buffer, otherwise it should be stored at -25℃ to -15℃ and protected from light. The validity period is 1 week, and repeated freezing and thawing should be avoided ( limited to 2 cycles).

4.After reconstituting the lyophilized positive control, it should be stored at -25°C to -15°C. The validity period is 1 week, and repeated freezing and thawing should be avoided (limited to 2 cycles).

5.Transportation conditions: It can be transported stably for 1 month when the temperature is not higher than 37℃.

6. See the packaging label for the production date, production batch number and expiration date.

 

[Applicable Instruments]

Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems(Shanghai Hongshi Medical Technology Co., Ltd. ), LightCycler®480 Real-Time PCR system, LineGene 9600 Plus Real-Time PCR Detection SystemFQD-96ABioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) , BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System.

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